Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells
서민석, 황경균, 김형범, 백승호,
소속 상세정보
서민석 ( Seo Min-Seock ) - Hanyang University College of Medicine Department of Dentistry
황경균 ( Hwang Kyung-Gyun ) - Hanyang University College of Medicine Department of Dentistry
김형범 ( Kim Hyong-Bum ) - Hanyang University College of Medicine Graduate School of Biomedical Science and Engineering
백승호 ( Baek Seung-Ho ) - Seoul National University School of Dentistry Department of Conservative Dentistry
KMID : 0362320120370030142
Abstract
Objectives: We analyzed gene-expression profiles after 14 day odontogenic induction of human dental pulp cells (DPCs) using a DNA microarray and sought candidate genes possibly associated with mineralization.
Materials and Methods: Induced human dental pulp cells were obtained by culturing DPCs in odontogenic induction medium (OM) for 14 day. Cells exposed to normal culture medium were used as controls. Total RNA was extracted from cells and analyzed by microarray analysis and the key results were confirmed selectively by reverse-transcriptase polymerase chain reaction (RT-PCR). We also performed a gene set enrichment analysis (GSEA) of the microarray data.
Results: Six hundred and five genes among the 47,320 probes on the BeadChip differed by a factor of more than two-fold in the induced cells. Of these, 217 genes were upregulated, and 388 were down-regulated. GSEA revealed that in the induced cells, genes implicated in Apoptosis and Signaling by wingless MMTV integration (Wnt) were significantly upregulated.
Conclusions: Genes implicated in Apoptosis and Signaling by Wnt are highly connected to the differentiation of dental pulp cells into odontoblast.
키워드
Differentiation; Gene set enrichment analysis; Human dental pulp cell; Microarray
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